N-hydroxyamobarbital-producing Bacillus-like Strains

Bacillus-like bacteria were screened for their N-hydroxyamobarbital productivities. Extracellular metabolites of 73 candidates were analyzed using the liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF MS) and Metlin database. The relative contents of N-hydroxyamobarbital in the culture media on strains that showed substantial levels were 6.04%, 4.85%, 1.61% and 1.61% for B. plakortidis FJAT-8757, B. endophyticus FJAT-10010, Aneurinibacillus migulanus FJAT-14205, and B. novalis FJAT-14227, respectively. The specialized m/z scores on FJAT-8757, FJAT-10010, FJAT-14205 and FJAT-14227 matched to ATPA were 96.25, 94.33, 96.72 and 94.91, respectively.