Optimized Extraction of Functional Ingredients from Callicarpa nudiflora Hook.et Arn.by Box-Behnken Design

To optimize the extraction of functional ingredients from the leaves of Callicarpa nudiflora Hook. et Arn., an experiment based on the Box-Behnken design was conducted. The extraction rate and contents of functional ingredients including flavonoids, phenolic acids, and saponins in the resulting extracts were weighed against the method, duration, cycle, material to solvent ratio, and ethanol concentration of the process. The weighted value for evaluation was calculated by the formula of(dry weight extract 0.7+ combined weight of functional ingredients 0.3). The optimized conditions included the application of ultrasound for 42 min, a material:solvent ratio of 1:17, the use of 62% ethanol for solvent, and two extraction cycles to arrive at a weighted value of 119.27, which was close to the predicted value of 120.47. The established extraction process was simple and applicable with desirable reproducibility and predictability.