Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

HE Hai-yan; QIU Hai-ping; CHAI Rong-yao; MAO Xue-qin; WANG Yan-li; SUN Guo-chang

doi:10.19303/j.issn.1008-0384.2019.02.012

Volume 34 Issue 2

Apr. 2019

Turn off MathJax

Article Contents

Abstract

References

Fujian Journal of Agricultural Sciences > 2019 > 34(2): 214-222. > DOI: 10.19303/j.issn.1008-0384.2019.02.012

HE Hai-yan, QIU Hai-ping, CHAI Rong-yao, MAO Xue-qin, WANG Yan-li, SUN Guo-chang. Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 214-222. DOI: 10.19303/j.issn.1008-0384.2019.02.012

Citation:

PDF (1427 KB)

Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

1.
Zhejiang Academy of Agricultural Sciences, Zhejiang Key Laboratory of Plant Pest Contro-Provincial and Ministerial Co-construction of National Key Laboratory Cultivation base, Hangzhou, Zhejiang 310021, China
2.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100080, China

More Information

Received Date: January 03, 2019
Revised Date: January 30, 2019

Graphical Abstract

Abstract

Abstract

Objective The distribution of 6 resistance genes in 40 cultivated rice varieties in Zhejiang (CRZ) and 6 physiological race identification (PRI) cultivars of Magnaporthe grisea as well as the blast resistance of the rice seedlings were studied.
Method The distribution of 6 resistance genes, i.e., Pi1, Pi9/Piz, Pi2/Pizt, Pikh, Pikm and Pita, in the rice varieties was obtained using functional markers. The resistance to M. grisea of all 46 rice varieties was determined based on the 141 strains collected in the field from 2015 to 2017.
Result Among the resistance genes, Pita had the highest distribution frequency at 47.83% followed by Pikh at 41.30%; Pi2/Pizt and Pikm, same at 34.78%; Pi9/Piz, low at 21.74%; and Pi1 found only in TTP, one of PRI cultivars. In all, there were 22 rice varieties that showed a resistance rate beyond 60%, and 2 varieties more than 80%.
Conclusion The resistance genes carried by the 40 CRZ and the 6 PRI cultivars partially correlated with the resistance level exhibited by the cultivars.
- rice,
- rice leaf blast,
- molecular marker,
- resistance gene

FullText(HTML)

References (31)

References

[1]	郑永利, 施德, 姚晓明, 等.晚稻稻瘟病局部突发流行成因与治理对策探讨[J].浙江农业科学, 2015, 56(1):92-93. http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029 ZHENG Y L, SHI D, YAO X M, et al. Discussion on the causes of local outbreak of rice blast in late rice and its control countermeasures[J]. Journal of Zhejiang Agricultural Sciences, 2015, 56(1):92-93.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029
[2]	谢子正, 许渭根, 李仁忠, 等. 2014年浙江省水稻稻瘟病流行特点及原因分析[J].中国植保导刊, 2015(3):58-60. DOI: 10.3969/j.issn.1672-6820.2015.03.014 XIE Z Z, XU W G, LI R Z, et al. Epidemiological characteristics and causes of rice blast in Zhejiang Province in 2014[J]. China Plant Protection, 2015(3):58-60.(in Chinese) DOI: 10.3969/j.issn.1672-6820.2015.03.014
[3]	向聪, 雷东阳, 任西明, 等.水稻抗稻瘟病遗传育种研究进展[J].作物研究, 2017(5):547-552. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022 XIANG C, LEI D Y, REN X M, et al. Research progress in genetic and breeding of rice blast resistance[J].Crop Research, 2017(5):547-552.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022
[4]	ANDERSEN J R, LUBBERSTED T.Functional markers in plants[J].Trends in Plant Science, 2003, 8(11):554-560. DOI: 10.1016/j.tplants.2003.09.010
[5]	谭令辞, 刘雄伦, 杨婷婷, 等.利用Pi9基因分子标记辅助选择培育抗稻瘟病水稻新品系[J].作物研究, 2015, 29(4):348-351. DOI: 10.3969/j.issn.1001-5280.2015.04.04 TAN L C, LIU X L, YANG T T, et al. Breeding new rice line with Blast Resistant by molecular marker-assisted selection of the Pi9 Gene[J].Crop Research, 2015, 29(4):348-351.(in Chinese) DOI: 10.3969/j.issn.1001-5280.2015.04.04
[6]	行璇, 刘雄伦, 陈海龙, 等.分子标记辅助选择Pi9基因改良R288的稻瘟病抗性[J].作物研究, 2016, 30(5):487-491. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002 XING X, LIU X L, CHEN H L, et al. Improving blast resistance of rice restorer R288 by molecular marker-assisted selection of Pi9 Gene[J].Crop Research, 2016, 30(5):487-491.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002
[7]	梁毅, 杨婷婷, 谭令辞, 等.水稻广谱抗瘟基因Pigm紧密连锁分子标记开发及其育种应用[J].杂交水稻, 2013, 28(4):63-74. http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022 LIANG Y, YANG T T, TAN L C, et al. Development of the molecular marker tightly-linked with the broad-spectrum blast resistance gene Pigm and its breeding practice in Rice[J]. Hybrid Rice, 2013, 28(4): 63-74.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022
[8]	杨丰宇, 李永聪, 刘雄伦, 等.分子标记辅助选择改良早籼稻1701的稻瘟病抗性[J].分子植物育种, 2017(6):2212-2217. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026 YANG F Y, LI YONGC, LIU X L, et al. Improving blast resistance of early indica rice 1701 by molecular marker-assisted selection for[J].Molecular Plant Breeding, 2017(6):2212-2217.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026
[9]	范方军, 王芳权, 刘永峰, 等. Pi-b、Pi-ta、Pikm和Pi54对水稻穗颈瘟的抗性评价[J].华北农学报, 2014, 29(3):221-226. http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040 FAN F J, WANG F Q, LIU Y F, et al. Evaluation of resistance to rice panicle blast with resistant genes Pi-b, Pi-ta, Pikm and Pi54[J].Acta Agriculturae Boreali-Sinica, 2014, 29(3):221-226.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040
[10]	宋兆强, 刘艳, 王宝祥, 等.稻瘟病抗性基因Pi-ta、Pi-b、Pi54和Pi-km的育种利用价值评价[J].江苏农业学报, 2017, 33(5):968-974. DOI: 10.3969/j.issn.1000-4440.2017.05.002 SONG Z Q, LIU Y, WANG B X, et al.[J]Application value of blast resistance genes Pi-ta, Pi-b, Pi54 and Pi-km in rice breeding[J]. Jiangsu Journal of Agricultural sciences, 2017, 33 (5): 968-974.(in Chinese) DOI: 10.3969/j.issn.1000-4440.2017.05.002
[11]	International Rice Research Institute. Standard Evalution System for Rice[M]. 4th ed. Manila: IRRI, 1996:17-18.
[12]	HUA L X, WU J Z, CHEN C X, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast[J]. Theoretical & Applied Genetics, 2012, 125(5):1047-1055. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=5fab0b7df029d28060cc59d64d6ece46
[13]	刘开强, 伍豪, 颜群, 等.水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J].西南农业学报, 2016, 29(6):1241-1244. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001 LIU K Q, WU H, YAN Q, et al. Development and application of specific molecular markers of blast resistance gene Pi1 in rice[J]. Southwest China Journal of Agricultural Sciences, 2016, 29 (6): 1241-1244.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001
[14]	QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9/Piz encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J].Genetics, 2006, 172(3): 1901-1914. DOI: 10.1534/genetics.105.044891
[15]	高利军, 高汉亮, 颜群, 等.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J].杂交水稻, 2010, 25(S1):294-298. http://d.old.wanfangdata.com.cn/Conference/7338573 GAO L J, GAO H L, YAN Q, et al. Establishment of markers for four blast genes and marker distribution in rice parents[J].Hybrid Rice, 2010, 25 (S1): 294-298.(in Chinese) http://d.old.wanfangdata.com.cn/Conference/7338573
[16]	ZHOU B, Qu S H, LIU G F, et al.The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions, 2006, 19(11): 1216-1228. DOI: 10.1094/MPMI-19-1216
[17]	SHARMA T R, MADHAY M S, SINGH B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-k (h) gene of rice, which confers resistance to Magnaporthe grisea[J]. Molecular genetics and genomics: MGG, 2005, 274(6): 569-578. DOI: 10.1007/s00438-005-0035-2
[18]	王军, 杨杰, 朱金燕, 等.稻瘟病抗病基因Pi-k^h功能标记的开发及江苏粳稻品种中Pi-k^h的变异[J].中国水稻科学, 2014, 28(2):141-14. DOI: 10.3969/j.issn.1001-7216.2014.02.005 WANG J, YANG J, ZHU J Y, et al. Development of a functional marker for rice blast resistance gene Pi-k^h and natural variation atPi-k^h in Japonica rice in Jiangsu province[J].Chinese Journal of Rice Science, 2014, 28 (2): 141-147.(in Chinese) DOI: 10.3969/j.issn.1001-7216.2014.02.005
[19]	ASHIKAWA I, HAYASHI N, YAMANE H, et al. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J]. Genetics, 2008, 180(4): 2267-2276. DOI: 10.1534/genetics.108.095034
[20]	徐小金.稻瘟病抗性基因资源的挖掘及其育种利用[D].金华: 浙江师范大学, 2016. XU X J. Exploitation and breeding application of rice blast resistance gene resources[D]. Jinhua: Zhejiang Normal University, 2016.(in Chinese)
[21]	BRYAN G T, WU K S, FARRALL L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. The Plant Cell Online, 2000, 12(11): 2033-2045. DOI: 10.1105/tpc.12.11.2033
[22]	JIA Y L, WANG Z H, SINGH P. Development of Dominant Rice Blast, Pi-ta, Resistance Gene Markers[J]. Crop Science, 2002, 42(6):2145-2149. DOI: 10.2135/cropsci2002.2145
[23]	JIANG H C, FENG Y T, BAO L, et al. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding[J]. Molecular Breeding, 2012, 30(4):1679-1688. DOI: 10.1007/s11032-012-9751-6
[24]	陈红旗, 陈宗祥, 倪深, 等.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学, 2008, 22(1):23-27. DOI: 10.3321/j.issn:1001-7216.2008.01.004 CHEN H Q, CHEN Z X, NI S, et al. Pyramiding three genes with resistance to blast by marker-assisted to improve rice blast resistance of Jin23B[J]. Chinese Journal of Rice Science, 2008, 22(1): 23-27.(in Chinese) DOI: 10.3321/j.issn:1001-7216.2008.01.004
[25]	柳武革, 王丰, 金素娟, 等.利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系稻瘟病抗性[J].作物学报, 2008, 34(7):1128-1136. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004 LIU W G, WANG F, JIN S J, et al. Improvement of rice blast resistance of TGMS Line by pyramising of Pi-1 and Pi-2 through molecular marker-assisted selection[J].Acta Agromomica Sinica, 2008, 34(7): 1128-1136.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004
[26]	DIVYA B, ROBIN S, RABINDRAN R, et al. Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43[J]. Euphytica, 2014, 200(1):79. DOI: 10.1007/s10681-014-1182-5
[27]	于苗苗, 戴正元, 潘存红, 等.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应[J].作物学报, 2013, 39(11):1927-1934. http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002 YU M M, DAI Z Y, PAN C H, et al. Resistance spectrum difference between two broad-spectrum blast resistance genes, Pigmand Pi2, and their interaction effect on Pi1[J].Acta Agromomica Sinica, 2013, 39 (11): 1927-1934.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002
[28]	邢鹏, 张幸, 李冬梅, 等.稻瘟病抗性基因在主要育种亲本中的分布研究[J].分子植物育种, 2015, 13(3):505-512. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004 XING P, ZHANG X, LI D M, et al. Research on the distribution of blast resistance genes in main rice breeding parents[J].Molecular Plant Breeding, 2015, 13(3): 505-512.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004
[29]	孙国昌, 杜新法, 陶荣祥, 等.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报, 1998(4):289-292. DOI: 10.3321/j.issn:0412-0914.1998.04.001 SUN G C, DU X F, TAO R X, et al. Control tactics and prospect of rice blast research in 21th century[J]. Acta Phytopathologica Sinica, 1998 (4): 289-292.(in Chinese) DOI: 10.3321/j.issn:0412-0914.1998.04.001
[30]	柴荣耀, 杜新法, 毛雪琴, 等.水稻不同类型品种上的稻瘟病菌群体小种的演变[J].浙江农业学报, 1999, 11(6):297-300. DOI: 10.3969/j.issn.1004-1524.1999.06.005 CHAI H, DU X F, MAO X Q, et al. Race evolution of Magnaporthe grisea on different rice varieties[J].Acta Agriculturae Zhejianggensis, 1999, 11(6): 297-300.(in Chinese) DOI: 10.3969/j.issn.1004-1524.1999.06.005
[31]	任鄄胜, 肖陪村, 陈勇, 等.水稻稻瘟病病菌研究进展[J].现代农业科学, 2008(1):19-23. http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019 REN J S, XIAO Y C, CHEN Y, et al. The research progress on rice blast fungus[J]. Modern Agricultural Science, 2008 (1): 19-23.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019

[1]	LU Xiaozhuan, ZHOU Shufen, LI Chenchen, CHEN Ziqiang, GUO Xinrui, LI Gang, TIAN Dagang. Molecular Marker-assisted Breeding of Sweet'n Waxy Corn[J]. Fujian Journal of Agricultural Sciences, 2024, 39(9): 1017-1022. DOI: 10.19303/j.issn.1008-0384.2024.09.002
[2]	PENG Jian, XIAO Youlun, YU Jianghui, ZHANG Yangjun, JIA Lin, TANG Xiaomei, LIU Jun, ZHOU Xiaoping, YU Cheng, LIU Jia. Codominance Functional Marker of Bacterial Blight Resistant Xa7 in Rice[J]. Fujian Journal of Agricultural Sciences, 2023, 38(4): 444-453. DOI: 10.19303/j.issn.1008-0384.2023.04.008
[3]	Deng Yun, Tian Dagang, Su Yan, Zhang Jiewei, Wu Jianwen. Physiological Races of Magnaporthe grisea and Disease-Resistant Rice in Fujian[J]. Fujian Journal of Agricultural Sciences, 2020, 35(10): 1101-1110. DOI: 10.19303/j.issn.1008-0384.2020.10.008
[4]	ZHANG Zhu-jian, CHEN Zi-qiang, GU Jian-qiang, TIAN Da-gang. Resistance on Rice Blast of Knockout Mutants of Pi-d2, Pi-d3 and Pigm[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1231-1236. DOI: 10.19303/j.issn.1008-0384.2018.12.001
[5]	ZHOU Peng, TU Shi-hang, DONG Rui-xia, WANG Hong-fei, ZHENG Yi, WANG Zhi-fu, ZHANG Shui-jin, YOU Qing-ru, DONG Lian-fei, HUANG Ting-xu, ZHENG Jia-tuan. Molecular Detection and Resistance Determination of Rice Blast-resistance Genes[J]. Fujian Journal of Agricultural Sciences, 2016, 31(9): 962-965. DOI: 10.19303/j.issn.1008-0384.2016.09.012
[6]	YANG De-wei, YE Ning, YE Xin-fu, ZHENG Xiang-hua, CHENG Chao-ping, LU Li-bin, HUANG Feng-huang. Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene[J]. Fujian Journal of Agricultural Sciences, 2015, 30(4): 351-356. DOI: 10.19303/j.issn.1008-0384.2015.04.008
[7]	LUO Ling-jie, ZHOU Yi-fei, KE Lan-lan, PAN Da-ren. Cloning and Analysis of NBS-LRR Type Resistance Gene Analogues in Vigna radiata[J]. Fujian Journal of Agricultural Sciences, 2014, 29(1): 35-39. DOI: 10.19303/j.issn.1008-0384.2014.01.008
[8]	WANG Cui, XIE Bao-gui, CHEN Liang-jun, KE Ting, KE Huo-zhao. SCAR Molecular Identification of 30 Flammulina velutipes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 966-970. DOI: 10.19303/j.issn.1008-0384.2013.10.007
[9]	LI Qi-xiang, ZHOU Yuan-chang, CHEN You-yu. Screening of Microsatellite Markers for Resistance to Bacterial Leaf Blight and Their Application to Gene Pyramiding Breeding in Rice[J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 470-474.
[10]	ZHU Yong-sheng, CAI Qiu-hua, XUE Wei-min, XIE Hong-guang, WU Fang-xi, ZHANG Jian-fu, XIE Hua-an. Preliminary Genotype Analysis of Broad-spectrum Rice Blast Resistance Variety Yunyin and its Near-isogenic Lines Construction[J]. Fujian Journal of Agricultural Sciences, 2012, 27(1): 1-6.

Cited By

Cited by

Periodical cited type(12)

1.	潘浩男，潘贞珍，环秀菊，覃潇敏，郑树芳，陆宇明，康专苗，陶亮，王文林，覃振师. 澳洲坚果桂热1号人工授粉有效性及花粉直感研究. 果树学报. 2025(01): 151-161 .
2.	孔广红，陶丽，贺熙勇，倪书邦，陈丽兰，肖晓明. 不同授粉组合对澳洲坚果良种HAES863坐果及果实大小的影响. 中国果树. 2024(03): 93-97 .
3.	孔广红，余文才，李玲，陶丽，贺熙勇. 云南西双版纳澳洲坚果开花与结实特性. 南方农业学报. 2024(04): 1089-1098 .
4.	马静，李扬，陶亮，贺熙勇，龙青姨，宫丽丹. 澳洲坚果种质资源花序表型性状多样性分析. 云南农业大学学报(自然科学). 2024(04): 131-140 .
5.	刘姚，张金云，吴世军. 广东省澳洲坚果种业发展现状与展望. 果树资源学报. 2023(01): 1-6 .
6.	万继锋，卢智强，邹明宏，陈菁，宋喜梅，杨倩，董丽红，罗炼芳，曾辉. 澳洲坚果成熟期间果实内果皮颜色、果仁品质变化及最适采收期预测. 中国南方果树. 2023(06): 72-75+84 .
7.	林玉虹，杨祥燕，蔡元保，潘如军，崔明勇，叶维雁，曾黎明，黄思婕，李冰. 68份澳洲坚果种质资源的表型分类研究. 南方农业学报. 2023(08): 2379-2387 .
8.	万继锋，曾辉，邹明宏，陈菁，宋喜梅，杨倩，罗炼芳，陆超忠. 澳洲坚果种质资源果实表型多样性分析. 中国南方果树. 2022(06): 134-140 .
9.	万继锋，曾辉，邹明宏，陈菁，罗炼芳，陆超忠. 八个澳洲坚果品种成年树抽梢、开花结果特性及栽培要点. 热带农业科学. 2021(03): 43-47 .
10.	谭秋锦，韦媛荣，黄锡云，张涛，许鹏，宋海云，王文林，郑树芳. 10份澳洲坚果种质果实性状与营养成分分析. 果树学报. 2021(05): 672-680 .
11.	万继锋，曾辉，邹明宏，陈菁，罗炼芳，陆超忠. 湛江地区澳洲坚果成年树抽梢生物学特性的观测分析. 中国南方果树. 2021(05): 65-70+75 .
12.	项亚男. 澳洲坚果形态解剖学及产业研究进展. 南方农业. 2021(26): 151-152 .

Other cited types(0)

Get Citation

PDF

XML

Article Metrics

Article views (1768) PDF downloads (34) Cited by(12)

Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

Abstract

References

Related Articles

Cited by

Periodical cited type(12)

Other cited types(0)

Catalog

Article Metrics

Related

Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

Abstract

References

Related Articles

Cited by

Periodical cited type(12)

Other cited types(0)

Catalog

Article Metrics

Related

Export File

Citation

Format

Content