Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754

  Objective  To construct a kinetic model for the optimized fermentation of Bacillus amyloliquefaciens FJAT-8754, which was capable of effectively producing cellulase and amylase.

  Method  A 50 L fermenter experiment was conducted to study the cellular growth, pH value, enzyme production and carbohydrate consumption of the fermentation and for subsequent process optimization. A kinetic model was established using the logistic and Luedeking-Piret equations, and experimental data calculated and analyzed with SPSS 16.0 software.

  Result  The optimal fermentation conditions fitted well in the non-linear model as shown by Origin 9.0 software.

  Conclusion  The kinetic model obtained accurately reflected the performance of the fermentation. The cellulase and amylase were products of FJAT-8754 metabolism in the culture.