Kinetics of Cellulase and Amylase-producing Fermentation of <i>Bacillus amyloliquefaciens</i> FJAT-8754

GE Ci-bin; LAN Jiang-lin; LIU Bo; WANG Kai; ZHENG Xue-fang

doi:10.19303/j.issn.1008-0384.2019.06.011

Volume 34 Issue 6

Sep. 2019

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Fujian Journal of Agricultural Sciences > 2019 > 34(6): 697-704. > DOI: 10.19303/j.issn.1008-0384.2019.06.011

GE Ci-bin, LAN Jiang-lin, LIU Bo, WANG Kai, ZHENG Xue-fang. Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 697-704. DOI: 10.19303/j.issn.1008-0384.2019.06.011

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Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754

Institute of Agricultural Bio-resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

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Received Date: December 10, 2018
Revised Date: March 24, 2019

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Abstract

Abstract

Objective To construct a kinetic model for the optimized fermentation of Bacillus amyloliquefaciens FJAT-8754, which was capable of effectively producing cellulase and amylase.
Method A 50 L fermenter experiment was conducted to study the cellular growth, pH value, enzyme production and carbohydrate consumption of the fermentation and for subsequent process optimization. A kinetic model was established using the logistic and Luedeking-Piret equations, and experimental data calculated and analyzed with SPSS 16.0 software.
Result The optimal fermentation conditions fitted well in the non-linear model as shown by Origin 9.0 software.
Conclusion The kinetic model obtained accurately reflected the performance of the fermentation. The cellulase and amylase were products of FJAT-8754 metabolism in the culture.
- Bacillus amyloliquefaciens,
- fermentation,
- kinetic model,
- cellulase,
- amylase

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Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754

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