Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells

  Objective  Effects of Laminaria japonicapolysaccharides on the proliferation and lipid peroxide production of H₂O₂-induced vascular smooth muscle cells (VSMCs) were examined in vitro for further understanding on the metabolic mechanism.

  Method  Proliferation of VSMCs was artificially induced by using 50 µmol·L⁻¹​​​​​ H₂O₂. Inhibitory rate on the proliferation by the L. japonica polysaccharides were determined by a methyl thiazolyl tetrazolium (MTT) assay. Morphological changes on VSMCs were observed under a microscope. Malondialdehyde (MDA) was employed as an indicator of the lipid peroxide production in VSMCs.

  Result  A significant inhibition effect of the polysaccharides on the H₂O₂-induced VSMC proliferation with the greatest rate of 73.56% was shown by MTT. Morphologically, the number of VSMCs significantly decreased after an exposure to the polysaccharide. The MDA analysis indicated that the lipid peroxide production by VSMCs significantly declined after the treatment with L. japonica polysaccharides.

  Conclusion  The L. japonica polysaccharides significantly inhibited the proliferation and lipid peroxide production in the H₂O₂-induced VSMCs.