Objective To identify conditions that facilitate germination of Mycogone perniciosa chlamydospores for further research on effective control of the fungal disease on edible mushrooms.
Method Chlamydospores were kept at −80℃, −20℃, 0℃, 4℃, 10℃, 15℃, 20℃ or 25℃ for various durations prior to culture on media at 25℃ to determine the effects of dormancy-breaking temperatures. And effects of varied pHs, culture conditions, carbon source and nitrogen source on the chlamydospores germination were detected.
Result Dormancy of the chlamydospores was broken and germination promoted under 0℃ or 4℃ for 12 h, 24 h or 48 h. pH 6, 7 and 8 were conducive to the spore germination at the rates of 8.66%, 9.79%, and 8.40%, respectively. The germination rate varied according to the medium the spores grew on. It was 0.00% on WA, 6.38% on MuEA, 8.43% on MuDA, 1.33% on PDA, and 6.23% on V8. Thus, MuDA appeared significantly better than other media tested in this study. The chlamydospores showed a germination rate of 10.77% with glucose, 9.96% with sucrose or 9.85% with sorbose in the medium, indicating a significantly advantage of the presence of a carbon source. In so far as nitrogen is concerned, the addition of proline in MuDA delivered a 12.32% germination rate, serine 13.45%, alanine 13.74%, and ornithine 11.64%, which were significantly improved over control without the addition.
Conclusion By keeping M. perniciosa chlamydospores at 0℃ or 4℃ for more than 12 h could break the spore dormancy. On MuDA of pH 6, 7 or 8, the chlamydospores germinated well. With the presences of glucose, sucrose, D-lactose, sorbic sugar or D-raffinose as carbon source and L-proline, L-serline, L-alanine or L-qrnithine as nitrogen source, the spore germination was further enhanced.
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