Objective Characteristics and full genomic sequence of Chilliveinal mottle virus (ChiVMV), one of the most recurring viral pathogens infecting chili pepper plants in South-East Asia were studied to decipher the evolution of the virus for improved estimation on its potential threat to Solanacae crops.
Method The ChiVMV gene from Hunan isolate (ChiVMVhn) was sequenced using the small RNA high-throughput sequencing and RT-PCR. Molecular characteristics of the gene were analyzed by Mega, RDP, and DnaSP software.
Result The gene of ChiVMVhn contained 9,704 nucleotides (excluding 3’-poly A) and shared 84%-94% identity with those from the other ChiVMVs. The phylogenetic analysis suggested that ChiVMVhn represented an evolutionary isolate distinctive from those reported previously elsewhere in the world. The nucleotide substitution in ChiVMVhn gene was R=3.29 and dominated by C/T.
Conclusion RNA mutation, rather than recombination, in the genes was seen crucial in the evolution of ChiVMVhn. Further studies on varieties of ChiVMV would considerably aid the scientific understanding on the evolution and pathogenic potential of the virus.
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