Process Optimization and Antimicrobial Activity of Natural Preservative Extracted from Bamboo Sawdust

HU Yong-le; ZHANG Chuan-hai; ZHANG Yuan; YOU Yong-fang; HUANG Wei; JIA Xiao-li

doi:10.19303/j.issn.1008-0384.2020.02.014

Volume 35 Issue 2

Feb. 2020

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Fujian Journal of Agricultural Sciences > 2020 > 35(2): 226-234. > DOI: 10.19303/j.issn.1008-0384.2020.02.014

HU Y L, ZHANG C H, ZHANG Y, et al. Process Optimization and Antimicrobial Activity of Natural Preservative Extracted from Bamboo Sawdust [J]. Fujian Journal of Agricultural Sciences，2020，35（2）：226−234. DOI: 10.19303/j.issn.1008-0384.2020.02.014

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Process Optimization and Antimicrobial Activity of Natural Preservative Extracted from Bamboo Sawdust

Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, College of Ecology and Resource Engineering, Wuyi University, Wuyishan, Fujian　354300, China

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Received Date: August 01, 2019
Revised Date: October 31, 2019

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Abstract

Objective Optimum preparation and antibacterial properties of a natural preservative extracted from bamboo sawdust were studied.
Method Utilizing bamboo sawdust, a natural preservative was prepared with the ethyl acetate reflux method. Processing conditions were optimized by the response surface methodology based on the results of a single factor test. In vitro antimicrobial activity of the extract was determined.
Result The finalized process applied 300 W ultrasonic power on the mixture of solvent-to-substrate ratio at 20 ∶ 1 to extract at 89℃ for 2 h. The product yield reached 4.77%. The extracted preservative displayed peak antimicrobial effects on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Aspergillus niger with the average inhibition diameters of 9.33 mm at a concentration of 0.025 00 g·mL⁻¹, 15.67 mm at a concentration of 0.012 5 0g·mL⁻¹, 10.00 mm at a concentration of 0.050 00 g·mL⁻¹, and 6.67 mm at a concentration of 0.100 00 g·mL⁻¹, respectively.
Conclusion The natural preservative prepared by the newly developed extraction method showed strong in vitro antimicrobial effect, and the optimized process could be upscaled to fully utilize the bamboo sawdust.
- Bamboo sawdust,
- natural preservative,
- response surface methodology,
- process optimization,
- antimicrobial property

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