Objective MYB14 in Longan was cloned, its bioinformatics studied, and expression vector constructed.
Method Full-length sequence of MYB14 was obtained by transcriptome sequencing and bioinformatics analysis. Accordingly, specific primers for the cloning were designed, bioinformatics on the open reading frame sequence established, and overexpression vector constructed.
Result In the TBP-like subfamily of MYB-related family of genes, MYB14 had an 831 bp ORF encoding 276 amino acids with one MYB binding site. It was a non-transmembrane hydrophilic protein with 57 amino acid phosphorylation sites in the cytoplasm. Its secondary structure was irregular crimp and α-helix. The predicted secondary and tertiary structures were highly consistent. The phylogenetic tree on the gene of longan showed a homology with that of cannabis.
Conclusion MYB14 of Longan was successfully cloned with the constructed expression vector, PBI121-MYB14, which provided reference for the further study on the function of MYB14.
DownLoad: