Visual LAMP for <i>Salmonella</i> Detection on Soil for Vegetable Farming

LYU Xin; LIU Lanying; CHEN Lihua; LI Yueren

doi:10.19303/j.issn.1008-0384.2021.02.015

Volume 36 Issue 2

Feb. 2021

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Fujian Journal of Agricultural Sciences > 2021 > 36(2): 236-242. > DOI: 10.19303/j.issn.1008-0384.2021.02.015

LYU X, LIU L Y, CHEN L H, et al. Visual LAMP for Salmonella Detection on Soil for Vegetable Farming [J]. Fujian Journal of Agricultural Sciences，2021，36（2）：236−242. DOI: 10.19303/j.issn.1008-0384.2021.02.015

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Visual LAMP for Salmonella Detection on Soil for Vegetable Farming

LYU Xin^{1, 2,},
LIU Lanying^{1, 2},
CHEN Lihua^{1, 2},
LI Yueren^{1, 2, ,}

1.
Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou, Fujian　350003, China

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Received Date: September 15, 2020
Revised Date: November 22, 2020
Available Online: February 07, 2021

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Abstract

Abstract

Objective A visual loop-mediated isothermal amplification (LAMP) technique for rapid detection of Salmonella in soil was developed to facilitate the prevention and control of the pathogens in vegetable fields.
Method Based on the sequence of the invasion protein A (invA) of Salmonella, LAMP primers were designed and reaction system optimized. Specificity and sensitivity of the methodology were challenged by artificial inoculation and verified by the National Standard Detection Method for Salmonella Detection in Vegetable Cultivation Soil.
Result The LAMP reacted positively on Salmonella but negatively on 6 non-Salmonella strains. It showed a minimum detection limit of 7 CFU·25 μL⁻¹ and a sensitivity of 4×10²CFU·g⁻¹ on the artificially inoculated Salmonella. Compared with the standard method, the LAMP exhibited same testing accuracy.
Conclusion The newly developed visual LAMP assay was rapid, accurate and sensitive for the detection of Salmonella in soil for vegetable farming.
- Loop-mediated isothermal amplification (LAMP),
- vegetable cultivation soil,
- Salmonella,
- invA

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