Objective Effects of a bio-organic fertilizer on physicochemical properties and microbial community in rhizosphere soil of a banana field infected by Fusarium wilt disease were studied.
Methods In a Fusarium wilt infected banana field, an random block design experiment on the application of a bio-organic fertilizer, Biofert, was conducted. Six months after banana seedlings were transplanted to the field, rhizosphere soil samples from lots with and without Biofert application were collected to determine the nutrient contents by chemical analysis and the microbial composition and diversity by high-throughput sequencing and bioinformatics analysis.
Results Compared to control, the application of Biofert increased pH by 14.85%, the total N by 25%, and total P by 19.04%, but decreased the total Fe by 2.62% in the soil, while lowered the incidence of Fusarium wilt on the plants by 75%. In the rhizosphere soil, the Biofert-treated lots showed the relative abundance of, other than an 18.49% decrease on Proteobacteria, increases by 43.84% on Ascomycota, 90.64% on Chytridiomycota, 293% on Penicillium, 212% on Gibellulopsis, and 1193% on Talaromyces, 39.81% on fungal Chao1 index, 38.43% on ACE, and 86.85% on Shannon index.
Conclusion Biofert application not only improved the soil quality but also significantly altered the structure and diversity of the microbial community in rhizosphere soil and contributed to the reduced incidence of banana wilt disease.
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