A Rapid Detection Method on RPA of Sweet Potato Leaf Curl Virus

  Objective  A rapid isothermal amplification method for detecting the recombinant enzyme polymerase (RPA) in sweet potato leaf curl virus (SPLCV) was established.

  Method  A set of primers for RPA detection was designed based on the AV1 gene selected from SPLCV sequence. Reaction conditions for the detection was optimized and verified for applicability.

  Result  The newly established methodology took merely 20m to complete the nucleic acid amplification at 39℃, which greatly improved the detection efficiency．More importantly, the method was specific toward SPLCV and free of any cross reaction with DNAs of other pathogens, such as TYLCD, SPFMV, SPVG, or SPCSV. It also showed a high repeatability and a minimum detection limit on RPA of 10 pg·μL⁻¹.

  Conclusion  The newly developed method for SPLCV identification based on the detection of RPA in sweet potatoes was rapid, accurate, specific, repeatable, and easy to operate in the field.