High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus

  Objective  The microbial community in rhizosphere soils of Chinese narcissuses was analyzed.

  Method   The Illumina Miseq high-throughput sequencing technology was used to obtain the relevant conserved gene regions of the microbes in soil specimens from Chinese narcissus planting lots. The community structures and distributions of dominant species of bacteria, fungi, and archaea in the rhizosphere soils were analyzed.

  Result   In total, 175 840 optimized sequences were obtained and clustered from the specimens into 2 680 representative OTUs with a 97% similarity. The dominant bacteria were Chloroflexi (30.86%) and Proteobacteria (20.67%). Among the fungi, Ascomycota (84.94%) significantly overshadowed the others, with Chaetomium globosum (28.15%) and Ascomycetes (25.01%) accounted for the greater proportions. On archaea, Thaumarchaeota (51.40%), Bathyarchaeota (25.98%), and Euryarchaeota (20.65%) were the major phyla that had 25.67% SCG and 12.93% acidophilic ammonia oxidizing members.

  Conclusion   The rhizosphere soils of Chinese narcissuses harbored diverse and rich microbial species. The information obtained would aid the development and utilization of the natural resources.