Sequence and Annotation of Colletotrichum fructicola N425 Genome on Tea Plant

  Objective  Sequence and annotation of Colletotrichum fructicola N425 genomefrom a diseased tea plant were determined, and primary virulence-related genes identified.

  Method   Whole genome of N425 was sequenced and assembled using the Illumina HiSeq2500 PE150 platform. Predicted protein structure and functional annotation of the genes were obtained using the NR, KEGG, and KOG databases.

  Results   The genome was approximately 56.3 Mbp in length with 53.2% of G+C, 10 157 protein-coding genes, and several types of non-protein coding sequences. Of the genes, 1 356 were annotated in the PHI database that included 140 as the loss of pathogenicity, 720 in the CAZy database, and 302 related to the secondary metabolites. Some of these genes were involved in the cAMP-PKA and MAPK cascade signaling pathways as well as other pathogenic processes, such as host cell wall degradation, which could be responsible for the virulence on tea plants.

  Conclusion   The sequence and annotation of whole C. fructicola N425 genome were successfully obtained, and probable virulence-related genes identified.