- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
ZENG L W，ZHANG X Y，LIN M，et al. Cloning and Prokaryotic Expressions of Cassava MebZIP27[J]. Fujian Journal of Agricultural Sciences，2025，40（1） ：1−9. DOI: 10.19303/j.issn.1008-0384.2025.01.001
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The Class A basic leucine zipper protein(bZIP), which regulates genes carrying ABA-responsive elements associated with the environmental adaptations as well as growth, development, and physiological metabolism of plants, in cassava was studied.
The Class A bZIP in cassava, MebZIP27, was cloned using RT-PCR technology to determine the functions of the gene transcription factors. Bioinformatic methods were applied to analyze properties of the gene and protein, fluorescence quantitative PCR employed to examine spatiotemporal expressions and responses to stress, and prokaryotic expression used to produce MebZIP27 protein.
MebZIP27 was in the 5th chromosome of cassava with a coding region of
Located in nucleus and belonged to the Class A bZIP gene family, MebZIP27 expressed most significantly in the storage roots of a cassava plant and by an ABA or H2O2 induction.
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