Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia

ZHOU Yanfang; WANG Xiuzhi; ZHANG Xiaomei; LUO Xiangwen; LIU Zehong; CUI Congcong; MENG Lingqiang; SUN Junling

doi:10.19303/j.issn.1008-0384.2024.07.009

Volume 39 Issue 7

Jul. 2024

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ZHOU Y F, WANG X Z, ZHANG X M, et al. Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia [J]. Fujian Journal of Agricultural Sciences，2024，39（7）：819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009

Citation:

ZHOU Y F, WANG X Z, ZHANG X M, et al. Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia [J]. Fujian Journal of Agricultural Sciences，2024，39（7）：819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009

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Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia

doi: 10.19303/j.issn.1008-0384.2024.07.009

1.
Chifeng Academy of Agricultural and Animal Science of Inner Mongolia, Chifeng, Inner Mongolia　024031, China
2.
Hunan Academy of Agricultural Science, Changsha, Hunan　410125, China
3.
Rural integrated Service Center of Gaofeng Tujia Village of Cili County of Hunan Province, Zhangjiajie, Hunan　427205, China

Received Date: 2023-10-28
Rev Recd Date: 2024-01-11

Available Online: 2024-08-15

Publish Date: 2024-07-28

Abstract

Abstract

Objective Viruses that infected chili pepper crops in Chifeng City, Inner Mongolia were identified, and their genetic relationship analyzed. Methods The 47 infected chili pepper plants at 4 major producing areas in Chifeng City were diagnosed, and viruses identified by sRNA high-throughput sequencing with bioinformatic analysis and RT-PCR. Fragments of RT-PCR were sequenced using the Sanger method to conduct a phylogenetic analysis with the MEGA software. Results Six viruses were identified by sRNA high-throughput sequencing. Of which, 4 were re-verified by RT-PCR. The dominant viruses infecting the 4 regions differed significantly. They were Broad bean wilt virus 2(BBWV2) and Potato virus Y(PVY) in Chengzi County of Songshang District, PVY and Pepper mild mottle virus(PMMoV) in Jinshan County of Kalaqin District, and BBWV2 in Ningcheng County and Xiqiao County of kalaqin District. When the infection was caused by multiple viruses, it was mostly by two major pathogens and had the highest rate of occurrence at 61.54%. The phylogenetic analysis on the sequenced nucleotides of the viruses showed distinctive genetic differences between PMMoV and BBWV2 and, possibly, PVY of a novel genetic type. Conclusion There are significant differences in the types of viruses, detection rates, and types of virus co-infections that occur in different chili production areas of Chifeng City in Inner Mongolia .
- Chifeng City of Inner Mongolia,
- chili pepper viral disease,
- sRNA sequencing,
- RT-PCR,
- multiple species infection,
- phylogenetic analysis

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