Studies on Classification and Antiserotypes of Monoclonal Antibodies Against Pseudomonas Solanacearum

Mouse marrow cell was hy bridized by immunized BA LB/C mouse spleen cell of pseudo monas solanacearum.Four hybridoma cell lines PSB24,PSB28,PSB46 and P SB56 ag ainst pseudo monas so lanacearum were established.The chromosomce numbers of the hybrido macell lines ranged from 98 to 104.The ELISA titers of the culture supenatants and the ascitic fluid were 102-103 and 105-106 respect ively.These McAbsbelonged to Ig G1 and IgMindividually.Each of these four McAbs was reacted individually with 157 pseudo monas solanacearum from different areas by ELISA.The results showed that four antigen deter minants and seven mono antisero types (McAbⅠ-Ⅶ)were distinguished from these strains.A mong them,the first type(Ⅰ)and the thir done(Ⅲ)is do minant types and large amount,distributed widely,but the seventh type(Ⅶ) was an undo minant one and small amount,distrbuted narrowly in Fujian province.