Studies on Classification and Mono Antiserotypes of Monoclonal Antibodies Against Xanthomonas Campestris PV. Oryzicoa
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Graphical Abstract
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Abstract
Mouse marrow cell was hybridized by immunized BALB/C mouse spleen cell of xanthomonas campestris pv. oryzicola. Five hybridoma cell lines,M23,M6,M17,M260and M29,secreting mono-clonal antibodies(McAbs)against xanthomonas campestris pv. oryzicola were established. Each of these five McAbs was reacted individtially with 128 xanthomonas campestris pv. oryzicola from different areas in ELISA. The result showed that five antigen determinants and seven mono antiserotypes(McAb Ⅰ-Ⅶ)were distinguished from these strains. The result reveals that it is possible to classify pathogenic bac-teria by McAbs.
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