Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging

Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase(GUS) without the promoter was introduced into Oryza sativa L.Minghui 86 by agrobacterium mediated transformation.The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging.GUS histochemical assay at different stages in different organs,such as root,stem,leave,anther,hull,embryo and emdospern,of 2653 rice promoter trap lines was performed.The results showed that 83 rice promoter lines were GUS positive.Of all tested,24 lines were expressed in endosperm(i.e.,0.91%) and 19 lines expressed in anther(i.e.,0.72%).Information collected on the rice promoter trap lines could be useful in identifying new rice genes.