The Pollen Cryopreservation of Citrullus lanutas (Thunb.) Mansfeld

The pollen of Citrullus lanutas (Thunb.) Mansf eld. can be stored in liquid nitrogen (-19 6℃) . The survival rate of cryopreserved pollen decreased with the increase of drying period. When using anther as material , the relationship between the survival rate of cryopreserved pollen and drying period can be described as a parabola curve. The survival rate was also affected by cooling and warming rate, rapid cooling and warming support higher survival rate. The cryopreserved pollens may go on the normal fertilization, and the plants fruit after pollination with the pollen.