Multiplication of Anabaena Azollae in Vitro

This experiment tried to isolate Andbaena azollae directly from Azolla leaf cavities after surface sterilization, then grew it on various culture media and conditions.More longer green-keeping periord of isolated Anabdena azollae was found in alien medium suplemented with organic N and C, micro-aerobic(1% O2, 99% N2)condition to compare with N-free medium and aerobic condition.The isolates underwent a limited multiplication in above growing condition but failed to continue the further multiplication.These cyanobacteria were antigenically identical to the endosybionts of Azolla.