Molecular Epidemiology of Muscovy Duck Reovirus

The primers were designed to amplify the S1 and S4 gene according to the muscovy duck reovirus(MDRV) sequencein Genbank. S1 and S4 gene of MDRV strains isolated in China were cloned and then sequenced and analyzed. The results of showed S4 and S1 gene was 992 bp and 819 bp in length, respectively; the nucleotide sequence of S1 segment shared 92.2%-96.7% identity in China strains, but 92.2%-96.7% identity with France strain, 89026; and 76%-78% identity with ARV strain, S1133. The nucleotide suquence of S4 segment shared 95.2%-99.3% idnentity in China stains, but 92.3%-94.5% identity with France strain, 89026, and 21.2%-21.5% identity with ARV strain,S1133. Phylogenetic analysis demonstrated that MDRV was quite different from ARV. The Ten MDRV strains isolated in China has no distinct difference, but they were different from 89026 (isolated in France). The study provide the basic research on the prevention with muscovy duck reovirusis.