Identification of Cry-type Gene of Bacillus thuringiensis Strain LSZ 9408

PCR and SDS-PAGE were used to identify the cry-type gene of Bacillus thuringiensis (Bt) strain LSZ 9408. PCR analysis showed that Bt LSZ 9408 strain contained cry1 and cry2 genes. Five pairs of general primers-Un1, Un2, Un3, Un4 and Un7/8 were used to amplify PCR, and then the amplification results were detected by SDS-PAGE. Primes Un1 and Un2 formed about 270 bp and 700 bp DNA fragments on agarose gel electrophoresis, while none PCR products was found with the other three pairs of primers. The results of SDS-PAGE also indicated that Bt LSZ 9408 possessed cry1 and cry2 genes, which coded 130kD and 65kD ICPs.