Optimization of Agaropectin Extraction with Response Surface Methodology

Agaropectin was extracted by EDTA-Na2, the content of sulfate group and total sugar were determined by barium sulfate turbidimetry and hydrochloric acid and phenol-sulfuric acid method, respectively. Based on single factor experiments, the extraction technique for agaropectin was optimized using response surface methodology, the mathematical regression model was established for predicting the value of sulfate group content plus total sugar for agaropectin (Y) at different levels of adding EDTA-Na2(A), treating time (B) and temperature (C). The optimal extraction parameters for agaropectin were determined as: adding EDTA-Na2 5.90 g·L-1, treating time 2.2 h at temperature of 53℃, respectively. With the optimal parameters, the content of sulfate group plus total sugar for agaropectin extracted was 84.00±0.04%, which was in good agreement with the predicted value(Y).