Development of PCR assay for detecting avian Pasteurella multocida

To establish a specific and sensitive detection methodology for avian Pasteurella multocida infection,PCR was applied.The 460 bp of the specific fragments was amplified from avian P.multocida genome.None could be obtained from Escherichia coli,Riemerella anatipestifer,Salmonella,Staphyloccocus aureus,Avian paramyxovirus type 1,Muscovy duck retrovirus or genome of duck.The sensitivity test indicated that a minimum of 1ngμL-1 of avian P.multocida genome DNA could be detected by the newly developed PCR method.