Factors affecting protein extraction from carrot seeds

Factors including the ratio of sample to solution(W:V),pH value,buffers,time,amount of PVP,media and SDS that might affect the extraction of proteins from carrot seeds were studied.The results showed that the extraction was significantly affected by the ratio of sample to solution,pH,buffers,time and medium composition.Based on the preliminary results,protein extraction from carrot seeds for 1.0 h at 4℃ in 50 mmolL-1 Tris-HCl buffer with a 1:10 ratio of sample to solution and pH 8.0 produced the best result.