Genetic diversity of Ralstonia solanacearum on peanuts in Fujian

Genomic fingerprints of 37 isolates of the pathogen Ralstonia solanacearum collected form peanuts in different areas in Fujian were analyzed by using RAPD primers.Nine primers were selected from 150 randomly selected primers with abundant polymorphisms.Genomic DNA from different isolate was amplified with the selected primers.Eighty five marked bands were produced on the agarose electrophoretic gel,of which 84 bands were polymorphic.The frequency of genetic diversity was 98.82%.Based on the genetic distance,a dendrogram was constructed with UPGMA.Thirty seven tested isolates were separated into 7 RAPD groups.Thirty one of them were separated into RG2 and RG6,with genetic distances ranging from 0.08 to 0.95.It is concluded that there were apparent differences among the isolates from different locations.There existed genetic similarity within the 37 tested isolates,due possibly to spreading of the isolates among areas in Fujian.