rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions

The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS.The complete sequences of ITS and 5.8S rDNA,as well as partial sequences of 18S rDNA and 26S rDNA,in the range of 696-711 bp for the seven samples were obtained.By comparing the sequences,it was apparent that the ITS sequences of R. ophiopogonis and R. liriopes from different regions varied.Our analytical results provided a basis for molecular identification of the species.