Effects of two vacination methods on lymphocytes and goblet cells in intestinal epithelium of Anguilla anguilla

Oral or injection immunization with Aeromonas hydrophila-ISCOMs was applied on Anguilla anguilla for the study.The numbers of lymphocytes and goblet cells in the intestinal epithelium of A.anguilla at days 1,7,14 and 21 after the vaccination were determined by using HE and AB-PAS staining.The results showed that the immunization changed the numbers of lymphocytes and goblet cells,and the antibody activity in serum became higher after day 7.It was found that the injection immunization produced a higher antibody activity than the oral application.