Four diarrhea pathogens in piglets on large farms

PCR and RT-PCR were used to investigate the infection caused by enteropathogenic Escherichia coli(EPEC),transmissible gastroenteritis virus of swine(TGEV),porcine epidemic diarrhea virus of swine(PEDV) and rotavirus of swine(RDV) in piglets from 16 large-scale swine farms in Fujian in 2009.The result showed that only one farm was infected with the 201bp band of E.coli K88 and the 510bp band of F18ab fimbriae of E.coli eliciting edema disease of piglet.EPEC was not found in other farms.The positive rate of EPEC was 6.25%.There was a farm,in which the 497bp band of TGEV was found with a positive rate of 6.25%.Three farms were found with the 854bp band of PEDV or 342bp band of RDV.Hence,the positive rates of TGEV and RDV were both 18.75%.It was concluded that the EPEC,TGEV,PEDV and RDV diarrhea infection continued to exist in piglets in the large-scale swine farms in Fujian.However,the infection showed up only in separate areas,and the rates were significantly reduced as compared to the previous reports.