Technology system of culture for asparagus officiralis L. anther

Experiments were conducted to study the callus inducement and green shoot differentiation of the anther of the breed "Gijnlim",Asparagus officinalis L. The results showed that the optimum asparagus callus induction could be obtained by inoculation on a 1/2 MS culture medium containing 2.0 mg NAAL-1,1.0 mg 6-BAL-1and 6% sucrose after storage at 4℃ for 5-7 days. Moreover,a higher induction rate could be accomplished with a 32℃ incubation for 5 days following the inoculation. Supplementation with 300 mg hydrolyzed caseinL-1in the MS medium also benefited the callus induction. Callus type and age affected the regeneration rate. The regeneration rate of the callus of the dense form was higher than that of the loose form. On the different callus ages (i.e.,10-50 days),30-to 40-day-olds had the highest green shoots regeneration rate. The microscopic examination showed that,among the green shoots from the loose callus,5.7% of the plants were haploids,65.1% diploids,11.9% tetraploids and 17.3% aneuploids; while among the green shoots from the dense callus,28.4% were haploids,45.3% diploids,9.6% tetraploids and 16.7% aneuploids; and,the haploids from the dense callus were more than those from the loose callus.