Studies on the induction and propagation for the Phalaenopsis "V31" of PLB

The effects of different basic media(MS、1/2MS、VW、White),different compounds(coconut milk,potato,banana and tryptone)and different 6-BA concentrations(0 mgL-1、0.5 mgL-1、1.0 mgL-1、1.5 mgL-1、2.0 mgL-1)were studied by adopting Phalaenopsis "V31" PLB for explant culture.The results indicated that the optimum induction combination of the Phalaenopsis "V31" PLB was MS+0.5 mgL-1 6-BA+0.2 mgL-1 NAA+4% sucrose+0.2% activated carbon+1.0% agar;the optimum propagation of the PLB was 1/2MS+1.0 mgL-1 6-BA+0.2 mgL-1 NAA+3% sucrose+0.2% activated carbon+1.0% agar.