- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
WU X M, YE M F, YU J Y, et al. Accumulation, subcellular distribution, and chemical forms of zinc in Myriophyllum spicatum L. [J]. Fujian Journal of Agricultural Sciences，2025，X（X）：1−11.
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To explore the enrichment and distribution characteristics of zinc in the body of Myriophyllum spicatum L.,
a hydroponic experiments was conducted to investigate its biomass, height, leaf epidermal cell variation and antioxidant enzyme activities. The subcellular distribution and chemical form of Zn in roots, stems and leaves were further analyzed.
The results showed that M. spicatum can survive under different concentrations of Zn treatments. At a zinc concentration of 50 mg·L−1, both plant height and biomass significantly increased by 22.87% and 10.06%, respectively, compared to the control group, while there was no significant difference in chlorophyll content and MDA content in roots and leaves compared to the control. However, the SOD activity in roots and leaves was significantly higher than that of the control. Under a zinc concentration of 200 mg·L−1, there was no significant difference in the plant height and biomass of M. spicatum compared to the control. However, the chlorophyll content was significantly lower than that of the control treatment. Additionally, the roots and leaves exhibited significantly higher MDA content and SOD activity than the control. The leaf epidermal cells of M. spicatum exhibited partial damage. After Zn treatment, the Zn contents in the roots, stems and leaves of M. spicatum were 55.48～242.44, 14.78～31.02 mg·kg−1 and 18.01～69.79 mg·kg−1, respectively. The zinc contents in various organs of Z1 and Z2 treatments were significantly higher than those of the control, and the zinc content in the roots was significantly higher than that in the leaves and stems. The subcellular distribution of Zn in M. spicatum is mainly located in the cell wall parts of leaves and stems, accounting for 32.56%～49.50% and 41.21%～43.52%, respectively. Under exogenous Zn treatment, the Zn in the roots of M. spicatum was mainly present in soluble fractions, comprising 32.10%～50.48% of the total. The Zn existed in NaCl-, HAc-, and HCl-extracted forms in leaves and stems of M. spicatum accounting for 77.00%～86.00% and 81.00%～86.38%, respectively.And Zn mainly existed in water and NaCl-extracted forms in roots, accounting for 59.71%～63.65%.
Therefore, M. spicatum is a plant with better Zn accumulation, with roots having a stronger capacity than stems and leaves. The main resistance mechanism underlying Zn accumulation and tolerance in M. spicatum might involve cell wall retention, vacuoles segregation (soluble components) and the presence of Zn in various low-reactivity forms.
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