SRAP analysis on variant lines of Jinxiu and Fenghuang yellow peach

Seven yellow peach samples,including Jinxiu,Fenghuang and their variant lines,were studied by means of the sequence-related amplified polymorphism(SRAP) molecular marker technology.By screening a total of 40 SRAP primer combinations,it was found that 2 combinations could positively identify the differential amplification between Fenghuang and its variant line Jinhuang,as they were genetically diversified.Subsequently,these specific marker bands were cloned,sequenced,analyzed and converted into stable sequence characterized amplified region(SCAR) markers using the corresponding SCAR primers.The SCAR markers from SRAP were suitable for rapid identification of the variant lines.The evidence of the genetic diversity was further verified by SCAR PCR.On the other hand,DNA polymorphism between Jinxiu and its variant lines was not detected.