Isolation and nitrite degradation ability of a denitrifying bacterium

Six strains of denitrifying bacteria were isolated from the mud in marine aquaculture ponds.Among them,one showed the strongest nitrite degradation capability.The strain was subsequently identified to be a Pseudomonas sp.and named JN1.Its optimum nitrite degradation conditions were determined in the lab to be at 1 000-4 000 mgL-1 sodium nitrite concentration,5%(v/v) bacterial inoculums and pH 6.5-7.5 with 100 mL of fill volume in a 250 mL flask and moderate aeration in the fermentation for 18-30 h.