Determination of Momordica's germplasm diversity by ISSR

The inter-simple sequence repeat(ISSR) method was applied to assess the genetic variations in 48 Momordica cultivars.From 96 ISSR primers 14 were employed on 48 samples.Out of 181 amplified bands,113 were found to be polymorphic(60.32%).The clustering analysis of ISSR results indicated that the genetic similarity indices were between 0.685 and 0.865.Thus,the genetic diversity,similarity and relationships among the cultivars were revealed providing a theoretical foundation for Momordica breeding in the future.