Effect of Hormone on Tissue Culture and Rapid Propagation of Trichosanthes kirilowii Maxim

Effect of hormone application on tissue culture of Trichosanthes kirilowii Maxim was investigated. Stems with axillary buds were used as explantlet,and MS for the basic medium. A vailable methods for the rapid propagation to clone T. kirilowii Maxim are reviewed.In the experiment, the young adventitious buds continuously appeared for 15 days in the culture medium of MS + 1.0 mgL-1 BA. In 30 days, the average number of shoots was 6.5, and the average shoot ratio was 81.25%. The excised plantlets could produce root 15 days after inoculation on the MS + 0.4 mg NAA L-1 medium. They continued to gradually develop into normal plants. A single plant had an average of 5 roots. The average rooting percentage was 100%. This rapid propagation method for T. kirilowii Maxim appeared to offer many benefits at low cost.