• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.
Citation: ZHU Xiao-lin, WANG Shao, CHEN Shao-ying, LIN Feng-qiang, CHENG Xiao-xia, CHEN Shi-long, ZHU Xiao-li, LI Zhao-long. Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain[J]. Fujian Journal of Agricultural Sciences, 2011, 26(6): 925-929.

Cloning and Bioinformatics Analysis of M Gene of Porcine Transmissible Gastroenteritis Virus Fujian Strain

More Information
  • Received Date: July 26, 2011
  • Revised Date: August 18, 2011
  • In this study, we report the cloning and sequencing of M gene of porcine transmissible gastroenteritis virus Fujian strain (TGEV-FJ), and analyze the homology, signal peptide, glycosylation sites, phosphorylation sites, secondary structure of M protein by using bioinformatics software. The complete M gene in TGEV-FJ strain was successfully amplified which has 789 bp, encoding 262 amino acids and then constructed into the recombinant plasmid pGEM-T-M. Sequential analysis and phylogenetic studies demonstrated that the TGEV M gene has highly conservative and consanguineous between TGEV-FJ strain and HN2002 strain. The prediction results showed that M protein in TGEV-FJ strain had a signal peptide cleavage site (ACG16-17ER), and 3 potential N-glycosylation sites,6 serine phosphorylation sites,1 threonine phosphorylation sites and 5 tyrosine phosphorylation sites. The secondary structure of M protein prediction showed that alpha helix, beta sheet and random coil were 11.59%, 44.93% and 43.48%, respectively. The results of the present study would lay some basic molecular biological foundation for prokaryotic expression of M gene in TGEV-FJ strain, which would subsequently facilitate further research for molecular diagnosis and gene engineering vaccines development.
  • ESCORS D,ORTEGO J,ENJUANES L,et al.The membrane M protein car boxy terminal binds to transmissible gastroenteritis coronavirus core and contributes to core stability[J].J Virol,2001,75(3):1312-1324.
    韩国全.猪传染性胃肠炎病毒SC-Y株M基因序列分析、原核表达研究及真核表达载体构建.四川:四川农业大学,2006.
  • Related Articles

    [1]LI Jidong, YANG Xiangyan, CAI Yuanbao, LI Mu, ZENG Liming, HUANG Sijie, WU Fumin, LIN Yuhong, ZHENG Wenwu, HUANG Jinyuan. Cloning and Bioinformatics Analysis of Protein Phosphatase Genes MiSTPP1 and MiSTPP4 from Macadamia integrifolia[J]. Fujian Journal of Agricultural Sciences, 2021, 36(2): 176-181. DOI: 10.19303/j.issn.1008-0384.2021.02.006
    [2]YIN Ya-jun, ZHANG Tao, WANG Ling, LU Hong-zhao, DU Wei-li. Cloning and Bioinformatic Analysis of Anthocyanidin Synthase Gene from Hanzhong Black Rice[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 124-129. DOI: 10.19303/j.issn.1008-0384.2017.02.004
    [3]LIU Wei-hua, LIN Zheng-chun, FENG Xin, LAI Zhong-xiong. Cloning and Bioinformatic Analysis of HOS1 Promoter from the 'Tianbao' Banana (Musa acuminata. AAA group)[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 820-825. DOI: 10.19303/j.issn.1008-0384.2016.08.006
    [4]LAI Cheng-chun, HUANG Xian-gui, GAN Huang-can, PAN Hong, FAN Li-Hua. Cloning and Bioinformatics of DFR Gene in Vitis davidii Foёx[J]. Fujian Journal of Agricultural Sciences, 2016, 31(7): 683-689. DOI: 10.19303/j.issn.1008-0384.2016.07.003
    [5]FANG Zhi-zhen, ZHOU Dan-rong, YE Xin-fu, JIANG Cui-cui, PAN Shao-lin. Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 136-140. DOI: 10.19303/j.issn.1008-0384.2015.02.006
    [6]LI You-juan, GE Jun-qing, LIN Tian-long. Cloning and Bioinformatics Analysis of ORF51 Gene of Anguillae Herpesvirus FJ Strain[J]. Fujian Journal of Agricultural Sciences, 2014, 29(4): 314-318. DOI: 10.19303/j.issn.1008-0384.2014.04.004
    [7]LIN Feng-qiang, CHEN Shao-ying, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, ZHU Xiao-li, LI Zhao-long. Cloning and Characteristic Analysis of M Class Genome Segments in Muscovy Duck Reovirus MW9710[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 951-956.
    [8]ZHANG Zhi-deng, LUO Tu-yan, RAO Qiu-hua, CAO Zhi-yun, ZHENG Teng. Glutamine Synthetase Gene Amplification by 3'RACE and Bioinformatics Analysis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 919-923.
    [9]WU Jing-jing, HONG Zhi-hong, ZHANG Ka, CHEN Hong-wen. Cloning and Bioinformatic Analysis of 6-Phogluconate Dehydrogenase Gene from Aspergillus oryzae[J]. Fujian Journal of Agricultural Sciences, 2012, 27(4): 394-399.
    [10]LIU Shu-ji, WU Cheng-ye, SHAN Chao, WANG Ai-rong, WANG Zong-hua. Bioinformatic analysis of angiotensin converting enzyme in Danio rerio[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 205-211.

Catalog

    Article Metrics

    Article views (2856) PDF downloads (861) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return