Isolation of Porcine Epidemic Diarrhea virus strain FJ-11A in Fujian Province and Sequence Analysis of the ORF3 Gene

Porcine epidemic diarrhea virus (PEDV) FJ-11A strain was isolated from piglets suffering from a severe diarrhea in a pig farm of Fujian Province using Vero cell.The PEDV ORF3 gene was obtained using reverse transcription-polymerase chain reaction (RT-PCR) method according to the sequence download from the GenBank.The results showed that the ORF3 gene of the FJ-11A strain was 675 bp, encoding an open reading frame (ORF) with 224 amino acids.Compared with the ORF3 gene published previously in GenBank by DNAstar software, the ORF3 sequence of FJ-11A strain had the highest nucleotide homology at 99.3% with CPF1074 and PFF513, isolating in South Korea, and also had 97.8% homology with vaccine strain CV777. Based on genetic evolutionary, the PEDV strains which isolated in China were in the same phylogenetic relationship, whereas the candidate vaccine strains (attenuated DR13 and CV777) were in the other phylogenic lineage.