Real-time PCR Analysis of Genes Induced with the Magnaporthe oryzae from Japonica Rice Yunyin

In early stage, we analysed the gene expression profile of broad-spectrum rice blast resistance japonica variety Yunyin induced by Mangnaporthe oryzae, and constructed the gene co-expression network based on the dynamic samples. Ten important genes based on this network were chosen in this study, and the expression pattern of 10 genes for japonica rice Yunyin inoculated with Mangnaporthe grisea was analyzed. The highest expression stages of the 10 genes were different, of which 8 genes reached the highest expression stage at 36 hours, and 3 genes encoding transcription factors reached the highest expression stage at 24 hours. Meanwhile, some of the 10 genes expression were up-regulated at first, and then inhibited. Others were inhibited at first, then increased, and declined at last. This work will help us further to define the role of japonica rice Yunyin in disease resistance.