• 中文核心期刊
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Volume 22 Issue 4
Mar.  2012
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Abstract
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SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
Citation: SONG Bing, LI You-fa, LIN Zhi-min, CHEN Bin, ZHENG Wei-wen. PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 346-349.
shu

PCR-DGGE and homological analyses on isolates of nitrogen-fixing paddy soil bacteria

  • 1.

    Biotech Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;

  • 2.

    College of Life Science, Fujian Agriculture and Forest University, Fuzhou, Fujian 350002, China

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  • Received Date: July 25, 2007
  • Revised Date: September 03, 2007
    Graphical Abstract
    • Abstract
  • Eight bacterial isolates,which originated from paddy fields in Fujian,were analyzed using 16SrDNA-PCR-DGGE.Through sequencing and Blast analysis,9 N2-fixing bacteria strains were believed to belong to Mesorhizobium sp.,Sphingomonas sp.,Rhizobium sp.,Bradyrhizobium sp.,Klebsiella sp.,Burkholderia cepacia,Pseudomonas sp.,Nocardioides and Rhodococcus sp.,due to their high homology with the related accessions in Genbank.
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    • References (1)
  • WATANABE K,KIDAMA Y,HARAYAMA S.Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting[J].Microbiol Methods,2001,44(3):253-262.
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