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Volume 22 Issue 3
Apr.  2012
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JIANG Fan, GAO Hui-ying, CHEN Xiu-ping, LI Tao, YANG Ling, ZHENG Shao-quan. Inter simple sequence repeats system optimization and fingerprinting for Longan[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 256-260.
Citation: JIANG Fan, GAO Hui-ying, CHEN Xiu-ping, LI Tao, YANG Ling, ZHENG Shao-quan. Inter simple sequence repeats system optimization and fingerprinting for Longan[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 256-260.
shu

Inter simple sequence repeats system optimization and fingerprinting for Longan

  • 1.

    Fujian Breeding Engineering Technology Research Center for Longan and Loquat, Fuzhou, Fujian 350013, China;

  • 2.

    Pomology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;

  • 3.

    Agricultural college, Hebei University of Engineering, Handan, Hebei 057150, China

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  • Received Date: June 07, 2007
  • Revised Date: August 09, 2007
    Graphical Abstract
    • Abstract
  • Factors affecting the Inter-Simple Sequence Repeats (ISSR) analysis of longan,such as annealing temperature and concentrations of template DNA,Mg2 + and dNTP were examined.The optimal ISSR-PCR reaction system (25 μL) was found to be: 20 ng template DNA,1.5 U Taq DNA polymerase,0.7 μmol·L-1 primer,2.0 mmol·L-1 Mg2+,and 0.2 mmol·L-1 dNTPs.Using the single primer (ISSR-13),fingerprints of 12 genotypes were constructed from different origins with their abundant polymorphism of DNA spectra.The results suggested that ISSR-PCR could be a suitable reaction system for studying longan genetic diversity.
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    • References (5)
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    余艳,陈海山,葛学军.简单重复序列区间(ISSR)引物反应条件优化与筛选[J].热带亚热带植物学报,2003,11(1):15-19.
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