Studies on culturing the isolated microspores of radish(Raphanus sativus L.)

The culturing technique of isolated microspore was studied by using of thirty cultivars of summer and autumntype radishas donor plants.The embryoids had been obtainedfrom four cultivars of autumn type radish.The results showed the genotype of donor plants played a decisive role in the culture of isolated microspore,somatic embryo yields were significantly increased by low temperature pretreatmentof flower buds.Furthermore,the optimumstage of microspore for the cultivation was uninuclease anaphase,maximum somatic embryo yields was obtained with the heat-shock treatment for 48h at 33℃.The 1/2 NLN-12 medium,containing 0.05 mg·L-16-BA,0.2 (mg·L-1)NAA,0.02 mg·L-1KT,5 mg·L-12,4-D,and 5 mg·L-1AgNO3,0.05 mg·L-1AC,was the optimal mediumfor somatic embryo inducement.