Detection of the antibody against <i>Riemerella anatipestifer</i> Serotype 2 by indirect enzyme-linked immunosorbent assay

CHENG Long-fei; SHI Shao-hua; FU Guang-hua; LI Wen-yang; PENG Chun-xiang; HUANG Yu; CHEN Pu-yan

Volume 21 Issue 2

Apr. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2006 > 21(2): 131-134

CHENG Long-fei, SHI Shao-hua, FU Guang-hua, LI Wen-yang, PENG Chun-xiang, HUANG Yu, CHEN Pu-yan. Detection of the antibody against Riemerella anatipestifer Serotype 2 by indirect enzyme-linked immunosorbent assay[J]. Fujian Journal of Agricultural Sciences, 2006, 21(2): 131-134.

Citation:

CHENG Long-fei, SHI Shao-hua, FU Guang-hua, LI Wen-yang, PENG Chun-xiang, HUANG Yu, CHEN Pu-yan. Detection of the antibody against Riemerella anatipestifer Serotype 2 by indirect enzyme-linked immunosorbent assay[J]. Fujian Journal of Agricultural Sciences, 2006, 21(2): 131-134.

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Detection of the antibody against Riemerella anatipestifer Serotype 2 by indirect enzyme-linked immunosorbent assay

1.
Animal Husbandary and Veterinary Institue, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China

Received Date: 2005-08-08
Publish Date: 2006-06-15

Abstract

Abstract

The Riemerella anatipestifer (RA)Serotype 2 treated with ultrasonic cracking was used as coated antigen, horseradish peroxidase-labelled rabbit-anti-duck IgG was taken as the second antibody, Tetramethyl Benzidine di hydrochloride (TMB) was used as coloring agent in this test.The indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibody against RA Serotype 2.This method was proved to be very specific, sensitive and repetitive.The result showed that the ducks treated with inactivated vaccine of RA Serotype 2 had specific antibody in their sera detected with indirect ELISA on the 7th day after vaccination.The titre of the antibody was up to peak on the 28th day after vaccination, and then declines, the titre of the antibody on the 56th day after vaccination was higher than that on the 14th day after vaccination.
- Riemerella anatipestifer Serotype 2,
- antibody detection,
- indirect ELISA

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References(2)

References

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