Content of amylose in rice (Indica sativa L.) changed by sense or antisense expression starch branching enzyme gene rbe1

Sense or antisense expression starch branching enzyme gene rbe1 had been introduced into rice restoring line Minhui81 (Indica Sativa L.) to improve rice starch quantity by Agrobacterium—mediated transformation.PCR and Southern hybridization showed that rice starch branching enzyme 1 gene had been integrated into ricegenome.Analysis of amylose content of T0 generation seeds showed that the content of amylose with sense expression starch branching enzyme 1 gene reduced compared with that of the non-transgenie Minhui 81, while the content of amylose with antisens expression starch branching enzyme 1 gene increased.