Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography

A method for the simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography was developed.The quantitative analysis on the vitamin content in fish oil was made by characterizing the retention time and spectral graph, and using the maximum absorbance wavelength for peak area.The results showed that the average recovery was 89.6%—95.0%,and variety was 3.6%—5.1%.So this method was suitable to be used in the determination on vitamin A, D and E.