Determination of some organic acids in the fruits of loquat by RP-HPLC

A RP-HPLC method with UV detector was established to determine the organic acids in the fruits of loquat.The chromatographic conditions were Waters μBondapakTMC18 (3- 9mm i.d×300mm, 10μm) used for column (at 30℃)with 0.04 mol·L-1 KH2PO4 -H3PO4 buffer (pH2.4) as mobile phase, and flow rate 0.8 ml·min-1, detector UV-214 nm.The correlation coefficients of the calibration graphs were 0.9993-0.9999.The averaged recoveries were 85% -102%, RSD5.05%.