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Volume 20 Issue 3
Apr.  2012
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GUO Gen-he, PAN Wei, SU De-sen, LAO Qiu-hua, HE Zhi-gang, CHEN Han-zhen. Determination of some organic acids in the fruits of loquat by RP-HPLC[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3): 198-201.
Citation: GUO Gen-he, PAN Wei, SU De-sen, LAO Qiu-hua, HE Zhi-gang, CHEN Han-zhen. Determination of some organic acids in the fruits of loquat by RP-HPLC[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3): 198-201.
shu

Determination of some organic acids in the fruits of loquat by RP-HPLC

  • 1.

    Central Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;

  • 2.

    Institute of Pomology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

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  • Received Date: September 05, 2004
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    • Abstract
  • A RP-HPLC method with UV detector was established to determine the organic acids in the fruits of loquat.The chromatographic conditions were Waters μBondapakTMC18 (3- 9mm i.d×300mm, 10μm) used for column (at 30℃)with 0.04 mol·L-1 KH2PO4 -H3PO4 buffer (pH2.4) as mobile phase, and flow rate 0.8 ml·min-1, detector UV-214 nm.The correlation coefficients of the calibration graphs were 0.9993-0.9999.The averaged recoveries were 85% -102%, RSD5.05%.
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  • 宋永康,郭嘉,潘葳.影响测定反相高效液相色谱流动相pH值的因素[J].现代科学仪器,2005(1):95-97.
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