Preparation and preliminary application of anti-CpTI antibody by nucleic acid immunization

Constructing pcDNA3.1-CpTI and then immunizing the Balb/c mice by the method of intramuscular injection, the special antibody was obtained.Its titer was more than 1: 800 and the correlation coefficient was 0.9961.And the result of assaying the modified CpTI transgenic rice approved that the antibody cold be used in the qualitative determination of expression by cpti transgenic rice.