Cloning and expression analysis of WRKY transcription factors in soybeans

WRKY transcription factor family is one of its large kinds that involve in the biotic and abiotic responses.GmWRKY cDNA sequence,which had a 92% homology with Arabidopsis thaliana transcription factor WRKY14,was obtained by RT-PCR.Its expression pattern in different tissues and under abiotic stresses was analysed by semi-quantitative RT-PCR.The result showed that GmWRKY expressed in soybean roots,stems,as well as,leaves,and the expression was strongly induced by abiotic stresses,such as aluminum,P deficiency and salt.It suggested that GmWRKY could play an important role in stress resistance of soybean under abiotic stresses.