Several factors for culture of isolating microspores of Chinese cabbage

The varieties of early-mature and middle-late-mature Chinese cabbage were used to study the influence of genotype and physioloyical status of donor plants on microspore embryogenesis and plant regeneration.The results showed that inducement ratio of the embryoid was significantly different among the different donor plants and the embryo productivity had no significant difference between the earty-mature varieties and middle-late-maturing ones.The temperature from three days before bud plucking to the plucking day was a key factor and 14℃/24℃ (night/day)was most suitable to induce microspore embryogenesis.The bud in full florescence and the donor plant with good nutritional status were also beneficial to induce microspore embryogenesis and plant regeneration.